Ama-Lateral flow assays (ama-LFA) kulula ukuwasebenzisa, amathuluzi okuxilonga alahlwayo angahlola ama-biomarker kumasampuli anjengamathe, igazi, umchamo, nokudla.Ukuhlola kunezinzuzo eziningi kunobunye ubuchwepheshe bokuxilonga okuhlanganisa:
❆ Ubulula: Ubulula bokusebenzisa lezi zivivinyo abufaniswe - mane ungeze amaconsi ambalwa embobeni yesampula futhi ufunde imiphumela yakho ngeso emizuzwini embalwa kamuva.
❆ Ezomnotho: Izivivinyo azibizi - ngokuvamile zingaphansi kwedola elilodwa ngokuhlolwa ngakunye okuzokwenziwa esikalini.
❆ Iqinile: Ukuhlola kungagcinwa endaweni eshisa kakhulu futhi kube nempilo yeshelufu yeminyaka eminingi.
Izigidigidi zamapheshana okuhlola akhiqizwa unyaka ngamunye ukuze kuhlonzwe izifo ezithathelwana ngocansi, izifo ezithwalwa omiyane, isifo sofuba, isifo sokusha kwesibindi, ukuhlolwa kokukhulelwa nokuzala, izimpawu zenhliziyo, ukuhlolwa kwe-cholesterol/lipid, izidakamizwa zokuhlukumeza, ukuhlonzwa kwezilwane, nokuphepha kokudla, phakathi abanye.
I-LFA yakhiwe iphedi yesampula, iphedi ye-conjugate, umugqa we-nitrocellulose oqukethe imigqa yokuhlola nokulawula, kanye nephedi yokufaka.Ingxenye ngayinye igqagqene okungenani ngo-1–2 mm okuvumela ukugeleza kwe-capillary okungavinjelwe kwesampula.
Ukuze usebenzise idivayisi, isampula yoketshezi njengegazi, i-serum, i-plasma, umchamo, amathe, noma okuqinile okuncibilikisiwe, yengezwa ngokuqondile kuphedi yesampula futhi yimbi ngokusebenzisa idivayisi yokugeleza yangemuva.Iphedi yesampula yenza isampula ibe namandla futhi ihlunge izinhlayiya ezingafunwa njengamaseli abomvu egazi.Isampula lingabese ligeleza lingavinjelwanga liye kuphedi ye-conjugate equkethe ama-nanoparticles anombala oqinile noma ama-fluorescent ane-antibody endaweni yawo.Lapho uketshezi lufika ku-conjugate pad, lezi nanoparticles ezomisiwe ziyakhululwa futhi zixutshwe nesampula.Uma kukhona abahlaziyi abaqondiwe kusampula elabonwa i-antibody, lezi zizobophezela esikhumbeni somzimba.Ama-nanoparticles aboshwe nge-analyte abe esegeleza kulwelwesi lwe-nitrocellulose futhi enqamula umugqa wokuhlola owodwa noma eminingi kanye nomugqa wokulawula.Ulayini wokuhlola (onelebuli engu-T esithombeni esingenhla) uwukufunda okuyinhloko kokuxilongwa futhi uqukethe amaprotheni angakwazi ukuhamba angabopha i-nanoparticle ukuze ukhiqize isignali ehlotshaniswa nokuba khona kohlaziyo kusampula.Uketshezi luyaqhubeka lugeleza lunqamule umucu luze lufike entanjeni yokulawula.Ulayini wokulawula (obhalwe ukuthi C esithombeni esingenhla) uqukethe ama-affinity ligands azobopha i-nanoparticle conjugate noma ngaphandle kwe-analyte ekhona esixazululweni sokuqinisekisa ukuthi i-assay isebenza kahle.Ngemuva komugqa wokulawula, uketshezi lugelezela kuphedi yokuxubha okudingekayo ukumunca lonke uketshezi lwesampula ukuze kuqinisekiswe ukuthi kukhona ukugeleza okungaguquki kuyo yonke imigqa yokuhlola nokulawula.Kwezinye izivivinyo, i-chase buffer isetshenziswa embobeni yesampula ngemva kokwethulwa kwesampula ukuze kuqinisekiswe ukuthi yonke isampuli ihanjiswa ngaphesheya komugqa.Uma yonke isampula isidlulile emigqeni yokuhlola nokulawula, ukuhlola sekuphelile futhi umsebenzisi angakwazi ukufunda imiphumela.
Isikhathi sokuhlaziya sincike ohlotsheni lwelwelwesi olusetshenziswa ekuhlolweni kokugeleza kwe-lateral (ulwelwesi olukhudlwana lugeleza ngokushesha kodwa ngokuvamile aluzweli kancane) futhi luvamise ukuqedwa ngaphansi kwemizuzu eyi-15.
Isikhathi sokuthumela: Nov-27-2021